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lar fluid present in capillary samples due to individual physiology
or the affects of unacceptable capillary sampling technique can
cause false hemoglobin results.
One of several pre analytical factors associated with technique
revolves around the size and action mode of the lancet and how it
is used. This and other factors associated with capillary sampling
technique can have an impact on the puncture, blood flow and
ultimately on the results obtained. Additional factors unrelated to
sampling technique, such as an individual's capillary circulation
status, must also be recognized as potential causes of false results.
Therefore when testing for hemoglobin using capillary blood as
the sample, confirmation of an unacceptable or unexpected result
is needed to rule out potential pre analytical factors as the cause.
This is particularly helpful when a cut off value is used, i.e. when
screening for nutritional status based on hemoglobin. Since the
individual performing the test may not always be aware of all the
potential pre analytical factors or even those that are directly or
indirectly associated with sampling techniques, a second finger
stick should be performed when wanting to rule out pre analyti-
cal factors as a cause of an unacceptable or unexpected result.
Performance of the second finger stick by a different individual
may aid in lessening the affect of the factors that could have been
technique related.
Always handle blood specimens with care, as they might be
infectious. Consult local environmental authorities for proper
disposal. Always wear protective gloves when handling blood
specimens. The microcuvette is for single use only.
Materials required
+ Analyzer
•
HemoCue Hb 201
•
HemoCue Hb 201 Microcuvettes
•
Lancet (for capillary samples)
•
Pipette or other transfer device (for venous, arterial or control
material samples)
•
Lint-free wipe (non-fraying)
Hydrophobic plastic or glass slide (for venous, arterial or control
material samples)
Quality Control
The HemoCue Hb 201 + Analyzer has an internal quality con-
trol, the "self test". Every time the analyser is turned on, it will
automatically verify the performance of the optronic unit of the
analyser. This test is performed every second hour if the analyser
remains switched on.
If quality control checks are required for regulatory reasons
they should be performed using liquid controls recommended by
HemoCue.
2
Expected values
Adult Males 130–170 g/L (13.0–17.0 g/dL, 8.1–10.5 mmol/L)
Adult Females 120–150 g/L (12.0–15.0 g/dL, 7.4–9.3 mmol/L)
Infants, after neonatal period 110–140 g/L (11.0–14.0 g/dL,
6.8–8.7 mmol/L)
Children, two years to teenage: gradual increase to adult normals.
Due to a wide range of conditions (dietary, geographical,
etc) which affect normal values, it is recommended that each
laboratory establish its own normal range.
Measuring range
0–256 g/L (0–25.6 g/dL, 0–15.9 mmol/L).
Results above 256 g/L (25.6 g/dL, 15.9 mmol/L) will be displayed
as HHH.
Limitations
HemoCue Hb 201 Microcuvettes are for In Vitro Diagnostic use
only. The HemoCue Hb 201 + Analyzer is only to be used together
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