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6.3 1:5 Dilution (5 mL Total Volume)
1. Press
repeatedly until the display reads:
2. Press
to enter selection
3. Attach the pipette tip to the tip cone
The pipettor is now ready to begin the 1:5 dilution (5 mL total volume).
The display indicates that the pipettor is ready to aspirate 4.00 mL.
4. Place the tip in the diluent and press the Start-button. The unit will aspirate the 4.00 mL.
The following display indicates that the unit is ready to aspirate 1.00 mL.
5. Place the tip in the sample solution and press the Start-button to aspirate 1.00 mL.
The next display indicates that the unit is ready to dispense the total
volume (5 mL) with blow-out.
6. Position the pipette tip to dispense onto the 3M Petrifilm Plate and press the Start-button. The unit will dispense the full amount from the tip.
The display now indicates that the unit is ready to repeat the dilution
sequence, beginning with the 4.0 mL aspiration.
7. Eject the disposable tip by pressing the tip ejector button twice, and repeat the series beginning with step 3.
d l
1.5 5 mL
d l
1
4.00 mL
5
SPEED
IN
d l
2
1.00 mL
5
SPEED
IN
d l
5.0 mL
5
SPEED
OUT
d l
1
4.00 mL
5
SPEED
IN
7
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